Journal: Cell
Article Title: A Dual-Mechanism Antibiotic Kills Gram-Negative Bacteria and Avoids Drug Resistance
doi: 10.1016/j.cell.2020.05.005
Figure Lengend Snippet: (A) BCP analysis of E. coli lptD4213 cells after 30 min of treatment with 1% DMSO, 6.3 μg/mL SCH-79797 (1× MIC), 2 μg/mL trimethoprim (10× MIC), 25 μg/mL nisin (2× MIC), or the combination of 2 μg/mL trimethoprim (10× MIC) and 25 μg/mL nisin (2× MIC). Cytological profiles were clustered by the first three principal components that account for at least 90% of the variance between samples. Cells were stained with DAPI, FM4–64, and SYTOX Green. Shown here are the merged images of DAPI (blue) and FM4–64 (red). Scale bar, 2 μm.
Article Snippet: Hierarchical-clustering of the cytological features was performed using the single-linkage method with MANOVA ( , MATLAB functions manova1, manovacluster).
Techniques: Staining